Industry: Molecular Biology / PCR and Electrophoresis / Cloning / Proteomics / NGS
Principles and uses
Low melting point (LM) agarose is derived by organic synthesis that generates methoxylated groups from the basic agarose structure. The main properties of this agarose are its low melting and gelling temperatures compared to standard agarose. The low melting temperature allows the recovery of undamaged nucleic acids at a temperature lower than their denaturation temperature. Low gelling ensures that the agarose will be in a liquid state in a temperature range in which gel manipulations can be performed without prior extraction of the DNA of the gel slice
Some important features are:
– Lower gel strength than standard agarose. Still, the gels can be easily manipulated.
– Greater clarity (gel transparency) than standard agarose gels.
– Great sieving capacity.
LM agarose is classified into three categories, depending on the degree of derivatization. Gel/melt temperatures and gel strength are the most important differences. Agarose LM is used in electrophoresis of DNA fragments = 1000 bp, enzymatic gel processing (digestion, ligation, PCR), preparative electrophoresis and analysis and recovery of large DNA fragments for other applications.
- Ash: <0.4%
- Sulfate: <0.12%
- Clarity: 1.5% (NTU) <4
- Gel strength: 1.5% (g / cm2)> 500
- Gelation temperature: 1.5% (ºC) 24-28
- Melting temperature: 1.5% (ºC) <65.5
- DNase / RNase activity: Not detected
- WEE: <0.12
- Humidity: <10%
- Gel background: Very low
- Color: White
- Appearance: Fine and homogeneous powder
- Temperature. Min: 2 ºC
- Temperature. Max: 25 ºC